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myh4  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank myh4
    (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative <t>Myh4</t> mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.
    Myh4, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myh4/anti-NCAM/bio_rxiv__64898__2026__04__20__719776-217-50-53
    Average 95 stars, based on 1 article reviews
    myh4 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles"

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles

    Journal: bioRxiv

    doi: 10.64898/2026.04.20.719776

    (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative Myh4 mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative Myh4 mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Immunostaining, Muscles, Injection, Saline, Quantitative RT-PCR, Expressing, Two Tailed Test

    Related Articles

    Incubation:

    Article Title: Fast Myosin Binding Protein‐C Is a Vital Regulator in Young and Aged Fast Skeletal Muscle Homeostasis
    Article Snippet: About 10–20 μg of each sample lysate was loaded onto 4%–20% precast polyacrylamide gels (BioRad, Mini‐Protean TGX, Cat #4561094) and electrophoresed at 80–100 V for 90 min. .. Subsequently, the samples were transferred onto nitrocellulose membranes and blocked in 5% nonfat dry milk for 1 h. Primary antibodies, including sMyBP‐C (WH0004604M1 and SAB3501005, Sigma‐Aldrich), fMyBP‐C (SAB2108180, Sigma‐Aldrich), Myh4 (BF‐F3, DSHB), MyoM1 (20360‐1‐AP, Proteintech), Mylpf (A24975, Antibodies.com ), Foxo1 (18592‐1‐AP, Proteintech), Ankrd2 (11821‐1‐AP, Proteintech), Cryab (MA5‐27708, Invitrogen) and phospho‐sMyBP‐C (S59/S62 and T190/T191, ProSci Inc., Order # PAS22457 and # PAS22459 ), were incubated overnight at 4°C. ..

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles
    Article Snippet: .. Briefly, sections were fixed in cold acetone (−20°C), blocked (5% goat serum/1% BSA in PBS with M.O.M. blocking reagent; Vector Laboratories), incubated with primary antibodies overnight at 4°C, and detected with Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific). . Primary antibodies against MYH7 (1:50, BA-D5, DSHB), MYH2 (1:100, SC-71, DSHB), MYH4 (1:100, BF-F3, DSHB), LAMININ (1:200, 4H8-2, Santa Cruz) were used. .. Images were acquired using a BIOREVO BZ-X800 microscope system and analyzed using the Hybrid Cell Count application (Keyence, Osaka, Japan) to determine fiber-type proportions and CSA.

    Article Title: Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity.
    Article Snippet: .. Samples were incubated with 5 μg/mL of mouse antibodies specific for Myh7 (type I fibers, BA-D5), Myh2 (type IIa fibers, SC71), and Myh4 (type IIb fibers, BF-F3), all purchased from the Developmental Studies Hybridoma Bank, together with rabbit anti-AnkB (1:100)27–30,32,33,52–54 or anti-dystrophin antibodies. .. Sections were then washed and incubated with donkey anti-rabbit IgG (H+L) Alexa FluorTM Plus 405 (A48258), in combination with goat anti-mouse IgG isotypespecific antibodies anti-IgG2b-Alexa FluorTM 568 (A-21144), anti-IgG1Alexa FluorTM 647 (A-21240), and anti-IgM- Alexa FluorTM 488 (A-21042) from ThermoFisher.

    Article Title: Fast Myosin Binding Protein-C Is a Vital Regulator in Young and Aged Fast Skeletal Muscle Homeostasis.
    Article Snippet: About 10–20 μg of each sample lysate was loaded onto 4%–20% precast polyacrylamide gels (BioRad, MiniProtean TGX, Cat #4561094) and electrophoresed at 80–100 V for 90 min. .. Subsequently, the samples were transferred onto nitrocellulose membranes and blocked in 5% nonfat dry milk for 1 h. Primary antibodies, including sMyBP- C (WH0004604M1 and SAB3501005, Sigma- Aldrich), fMyBP- C (SAB2108180, Sigma- Aldrich), Myh4 (BF- F3, DSHB), MyoM1 (20360- 1- AP, Proteintech), Mylpf (A24975, Antibodies.com), Foxo1 (18592- 1- AP, Proteintech), Ankrd2 (11821- 1- AP, Proteintech), Cryab (MA5- 27708, Invitrogen) and phospho- sMyBP- C (S59/S62 and T190/T191, ProSci Inc., Order # PAS22457 and #PAS22459), were incubated overnight at 4°C. ..

    Blocking Assay:

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles
    Article Snippet: .. Briefly, sections were fixed in cold acetone (−20°C), blocked (5% goat serum/1% BSA in PBS with M.O.M. blocking reagent; Vector Laboratories), incubated with primary antibodies overnight at 4°C, and detected with Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific). . Primary antibodies against MYH7 (1:50, BA-D5, DSHB), MYH2 (1:100, SC-71, DSHB), MYH4 (1:100, BF-F3, DSHB), LAMININ (1:200, 4H8-2, Santa Cruz) were used. .. Images were acquired using a BIOREVO BZ-X800 microscope system and analyzed using the Hybrid Cell Count application (Keyence, Osaka, Japan) to determine fiber-type proportions and CSA.

    Immunolabeling:

    Article Title: A mouse model of CHCHD10 p.R15L familial ALS presents mild, age-related motor neuron degeneration without protein instability or mitochondrial dysfunction
    Article Snippet: .. 10 μm thick cryostat sections were post fixed in acetone and immunolabeled with the following antibodies: laminin (1:200, rabbit, Sigma, L9393), Myh4 (1:50, mouse, DSHB BF-F3). .. Secondary antibodies diluted 1:500 in 10% NGS and 1% BSA in PBS were: Alexa Fluor 647 goat anti-rabbit and Alexa Fluor 555 goat anti-mouse IgM (Thermo Fisher Scientific).



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    (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative <t>Myh4</t> mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.
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    (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative <t>Myh4</t> mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.
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    (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative <t>Myh4</t> mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.
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    Image Search Results


    (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative Myh4 mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: bioRxiv

    Article Title: Cardiac-Detargeted MyoAAV Enables Systemic Nrl-Mediated Fast Myofiber Remodeling and Hypertrophy Across Multiple Skeletal Muscles

    doi: 10.64898/2026.04.20.719776

    Figure Lengend Snippet: (A–C) MyHC immunostaining using fiber-type-specific antibodies in the EDL (A), triceps (B), and soleus (C) muscles of TKO mice intravenously injected with saline (CON) or MyoAAV-Nrl-miR208a. Type I fibers are shown in blue, type IIa fibers in red, and type IIb fibers in green. Unstained fibers were classified as type IIx fibers (black). Scale bars are shown in the indicated images. (D–F) The proportion of each fiber type in the EDL (D), triceps (E), and soleus (F) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). (G–I) RT-qPCR analysis of relative Myh4 mRNA expression in the EDL (G), triceps (H), and soleus (I) muscles from TKO mice in the CON and MyoAAV-Nrl-miR208a groups (n = 9 and 8, respectively). P values were calculated using an unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Briefly, sections were fixed in cold acetone (−20°C), blocked (5% goat serum/1% BSA in PBS with M.O.M. blocking reagent; Vector Laboratories), incubated with primary antibodies overnight at 4°C, and detected with Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific). . Primary antibodies against MYH7 (1:50, BA-D5, DSHB), MYH2 (1:100, SC-71, DSHB), MYH4 (1:100, BF-F3, DSHB), LAMININ (1:200, 4H8-2, Santa Cruz) were used.

    Techniques: Immunostaining, Muscles, Injection, Saline, Quantitative RT-PCR, Expressing, Two Tailed Test